Journal: Scientific Reports

Article Title: Mining gut microbiome oligopeptides by functional metaproteome display

doi: 10.1038/srep34337

Figure Lengend Snippet: Oligopeptide:pIII fusion protein was stained with serum pools (1:200 dilution) from TNF ΔARE/+ and wildtype mice. Bound antibodies were detected with an anti-mouse (IgA, IgG, IgM) secondary antibody. Control staining of mouse anti-hexahistidine and exemplary mouse anti-pIII (JOZ158-E11) staining was included to identify the pIII fusion protein.

Article Snippet: Oligopeptide phage particles were produced for 24 h at 30 °C and 250 rpm (Infors HT).

Techniques: Staining, Control

Journal: Scientific Reports

Article Title: Mining gut microbiome oligopeptides by functional metaproteome display

doi: 10.1038/srep34337

Figure Lengend Snippet: Reactivity of TNF ΔARE/+ and wildtype sera with ( A ) JOZ158-E11 oligopeptide ( B ) JOZ158-G8 oligopeptide ( C ) JOZ156-H5 oligopeptide. The median cohort reactivity is indicated by a black line. Selected peptides were immobilized by streptavidin and bound serum antibodies from single sera (1:100 dilution) were detected with an anti-mouse (IgA, IgG, IgM) antibody. (Non-parametric Mann-Whitney test; ** = p < 0.01; *** = p < 0.001)).

Article Snippet: Oligopeptide phage particles were produced for 24 h at 30 °C and 250 rpm (Infors HT).

Techniques: MANN-WHITNEY

Journal: Scientific Reports

Article Title: Mining gut microbiome oligopeptides by functional metaproteome display

doi: 10.1038/srep34337

Figure Lengend Snippet: Antibody response of mice sacrificed at life week 4, 6, 8, 12 and 18. ( A ) Histologic scoring of ileal inflammation (only 2 out of 10 wildtype samples were scored); serum reactivity with ( B ) JOZ158-E11 oligopeptide ( C ) JOZ158-G8 oligopeptide ( D ) JOZ156-H5 oligopeptide. The median cohort reactivity is indicated by a black line. Selected peptides were immobilized by streptavidin and bound serum antibodies from single sera (1:100 dilution) were detected with an anti-mouse (IgA, IgG, IgM) antibody. (Non-parametric Mann-Whitney test; * = p < 0.05; ** = p < 0.01; ***p < 0.001).

Article Snippet: Oligopeptide phage particles were produced for 24 h at 30 °C and 250 rpm (Infors HT).

Techniques: MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Harnessing filamentous phages for enhanced stroke recovery

doi: 10.3389/fimmu.2023.1343788

Figure Lengend Snippet: (A) Illustration depicting the overall architecture of the M13 phage. (B) Schematic representation of the phage display biopanning process. A DNA library, either natural or synthetic, is inserted between the signal peptide and pIII encoding gene on a phagemid vector. The phagemid pool is introduced into Escherichia coli cells already infected with a helper phage. A subset of these capsids carries the inserted peptides. The phage library then undergoes iterative rounds of biopanning. Adapted from .

Article Snippet: Abundant viral-like particles (VLPs) like M13 phages offer cost-effective reservoirs for tailored drug packaging and precise targeting in nanocarrier-based drug delivery systems, ensuring biocompatibility and uptake efficiency ( , , , ).

Techniques: Plasmid Preparation, Infection

Journal: Frontiers in Immunology

Article Title: Harnessing filamentous phages for enhanced stroke recovery

doi: 10.3389/fimmu.2023.1343788

Figure Lengend Snippet: Illustrating diverse artificial cellular niches utilizing M13 phage as bionanomaterial. (A) Visualization of traditional biomaterial-infused scaffold featuring phage-displayed peptides and implanted stem cells to engineer a niche. (B) Depiction of biomimetic phage-infused scaffold encompassing implanted stem cells to engineer a niche. Adapted from .

Article Snippet: Abundant viral-like particles (VLPs) like M13 phages offer cost-effective reservoirs for tailored drug packaging and precise targeting in nanocarrier-based drug delivery systems, ensuring biocompatibility and uptake efficiency ( , , , ).

Techniques: